준 수직각형 로터

준 수직각형 로터(Near-vertical rotor)는 짧은 경로 길이, 빠른 실행 시간 및 탁월한 해상도를 특징으로 합니다.

준 수직각형로터에서 튜브는 수직에서 7.5°– 9° 정도 약간 기울어져 있습니다. 이러한 특징 때문에 샘플의 이동경로가 짧아집니다. 이동경로가 짧을수록 분리 시간도 짧아집니다.

약간의 각도는 펠릿이 튜브의 밑 면쪽으로 쌓이게 하여 실행이 멈추면 용액이 펠릿과 접촉되지 않습니다.

MLN-80

최대 RPM - 80,000
최대 RCF - 389,000 x g
K 계수 - 20
튜브 개수, 용량, 크기
- 8 X 8 mL
- 16 X 58 mm
로터 최대 용량 - 64 mL

NVT 100

최대 RPM - 100,000
최대 RCF - 750,000 x g
K 계수 - 8
튜브 개수, 용량, 크기
- 8 X 5.1 mL
- 1/2 X 2 in.
- 13 X 51 mm
로터 최대 용량 - 40.8 mL

NVT 65

최대 RPM - 65,000
최대 RCF - 402,000 x g
K 계수 - 21
튜브 개수, 용량, 크기
- 8 X 13.5 mL
- 5/8 X 3 in.
- 16 X 76 mm
로터 최대 용량 - 108 mL

NVT 65.2

최대 RPM - 65,000
최대 RCF - 416,000 x g
K 계수 - 15
튜브 개수, 용량, 크기
- 16 X 5.1 mL
- 1/2 X 2 in.
- 13 X 51 mm
로터 최대 용량 - 81.6 mL

NVT 90

최대 RPM - 90,000
최대 RCF - 645,000 x g
K 계수 - 10
튜브 개수, 용량, 크기
- 8 X 5.1 mL
- 1/2 X 2 in.
- 13 X 51 mm
로터 최대 용량 - 40.8 mL

TLN-100

최대 RPM - 100,000
최대 RCF - 450,000 x g
K 계수 - 14
튜브 개수, 용량, 크기
- 8 X 3.9 mL
- 13 X 38 mm
로터 최대 용량 - 31.2 mL

TLN-120

최대 RPM - 120,000
최대 RCF - 585,000 x g
K 계수 - 7
튜브 개수, 용량, 크기
- 8 X 1.2 mL
- 8 X 35 mm
로터 최대 용량 - 9.6 mL

FAQ on Near-Vertical Rotors

What is the difference between a near-vertical rotor and a fixed-angle rotor?

A fixed-angle rotor is typically optimized for rapid pelleting applications, while a near-vertical rotor is often chosen for density gradient and isopycnic centrifugation workflows. Near-vertical rotors provide a shorter path length within the gradient, which can help reduce separation times for equilibrium and gradient-based applications.

Why are near-vertical rotors used for density gradient centrifugation?

Near-vertical rotors provide a shorter sedimentation path than many other rotor designs. This can reduce run times and support efficient density gradient separations while maintaining resolution between particles with different buoyant densities.

Why choose a near-vertical rotor for DNA purification?

Near-vertical rotors are commonly used in density gradient-based nucleic acid separation workflows because they can support efficient migration of DNA molecules through the gradient while reducing run times compared with some traditional rotor configurations.

What is a near-vertical rotor?

A near-vertical rotor is an ultracentrifuge rotor that holds sample tubes at a shallow angle between vertical and fixed-angle orientations. This geometry creates a shorter sedimentation path, helping reduce centrifugation times while maintaining high separation efficiency.

What applications are near-vertical rotors commonly used for?

Near-vertical rotors are frequently used for density gradient centrifugation, isopycnic separations, DNA purification, viral particle isolation, lipoprotein analysis, and other high-resolution ultracentrifugation workflows.

What is isopycnic centrifugation?

Isopycnic centrifugation is a density gradient separation technique in which particles migrate until they reach a position where their density matches the density of the surrounding gradient medium. Near-vertical rotors are commonly used for these applications because they can support efficient equilibrium separations.